1 experimental dataset deposited supporting this annotation
  Protein Name  vimentin
  Post-translational Modification Type   Phosphorylation

  1.            Platform: Mass spectrometry
Experimental Details - HuPA_00031
Experiment type Mass spectrometry
Short description Phosphoproteome analysis
Experimental description 100ug of total cell lysate was run on the SDS-PAGE and the whole lane was in-gel digested with trypsin. From the exracted peptide mixture, phosphopeptides were enriched with TiO2.
Principal Investigator's NameDr. Blagoy Blagoev
TitleAssistant Professor
AddressUniversity of Southern Denmark
Institute of Biochemistry and Molecular Biology, Campusvej 55,
Odense M, DK- 5230
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue: Mesenchymal cell [eVOC:EV:0200171]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique None
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation Yes
Mass spectrometer
Instrument: LTQ FT [PSI:1000448]
Vendor: ThermoFinnigan [PSI:1000125]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 10 ppm
Mass tolerance used for database searching (MS/MS) 0.6 Da
Database used for searching IPI
Search engine used Mascot
Download MS raw dataset  
Download processed files  

   vimentin - Phosphorylation [RESID:AA0037]
  Peptide SequenceModificationsPeptide Score/ProbabilityPrecursor MassCharge StateSequence IdentifierMS/MS Spectrum
1. R.QDVDNASLAR.L Phosphorylation: S7
45 584.752062 2 IPI00418471.5 HuPA_S44254
2. R.QDVDNASLAR.L Phosphorylation: S7
24 576.240895 2 IPI00418471.5 HuPA_S44253
3. R.QDVDNASLAR.L Phosphorylation: S7
17 576.240895 2 IPI00418471.5 HuPA_S44252

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