HUMAN PROTEINPEDIA 


  1 experimental dataset deposited supporting this annotation
  Protein Name  plectin 1, intermediate filament bindingprotein 500kDa
  Post-translational Modification Type   Phosphorylation

  1.            Platform: Mass spectrometry
 
Experimental Details - HuPA_00015
Experiment type Mass spectrometry
Short description HeLa phosphoproteome anlaysis
Experimental description HeLa cells were stimulated with epidermal growth factor (EGF) and temporal profiles were observed.
Principal Investigator's NameDr. Matthias Mann
TitleProfessor
E-mailmmann@biochem.mpg.de
AddressMax Planck Institute for Biochemistry
Department of Proteomics and Signal Transduction
Martinsried, D- 82152
CountryGermany
Lab URLhttp://www.biochem.mpg.de/en/research/rd/mann/people/index.html
Data submitted byFlorian Gnad
TitlePh.D. Student
E-mailgnad@biochem.mpg.de
Published/Unpublished Published
Journal name
PubMed ID 17081983
Sample source
Tissue:
Cell line: HeLa
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique SILAC labeling method (L-lysine-2H4, L-lysine-U-13C6-15N2, L-arginine-U-13C6, L-arginine-U-13C6-15N4)
Protease used Trypsin
Is the sample from in gel No
Reduction and Alkylation DTT and iodoacetamide
Mass spectrometer
Instrument: 1. LTQ FT [PSI:1000448]
2. LTQ Orbitrap [PSI:1000449]
Vendor: 1. ThermoFinnigan [PSI:1000125]
2. ThermoFinnigan [PSI:1000125]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS)
Mass tolerance used for database searching (MS/MS)
Database used for searching IPI
Search engine used Mascot
Download MS raw dataset  
Download processed files  

   plectin 1, intermediate filament bindingprotein 500kDa - Phosphorylation [RESID:AA0037]
  Peptide SequenceModificationsPeptide Score/ProbabilityPrecursor MassCharge StateSequence IdentifierMS/MS Spectrum
1. TSSEDNLYLAVLR Phosphorylation: T1
Phosphorylation: S2
Phosphorylation: S3
106.25 IPI00398779  
2. TSSEDNLYLAVLR Phosphorylation: T1
Phosphorylation: S2
Phosphorylation: S3
101.78 IPI00398779  
3. TSSEDNLYLAVLR Phosphorylation: T1
Phosphorylation: S2
Phosphorylation: S3
101.78 IPI00398779  
4. KRTSSEDNLYLAVLR Phosphorylation: S4
Phosphorylation: S5
82.62 IPI00398779  
5. TSSEDNLYLAVLR Phosphorylation: T1
Phosphorylation: S2
Phosphorylation: S3
81.57 IPI00398779  
6. TSSEDNLYLAVLR Phosphorylation: T1
Phosphorylation: S2
Phosphorylation: S3
67.05 IPI00398779  
7. TSSEDNLYLAVLR Phosphorylation: T1
Phosphorylation: S2
Phosphorylation: S3
67.05 IPI00398779  
8. TSSEDNLYLAVLR Phosphorylation: T1
Phosphorylation: S2
Phosphorylation: S3
67.05 IPI00398779  
9. TSSEDNLYLAVLR Phosphorylation: T1
Phosphorylation: S2
Phosphorylation: S3
56.67 IPI00398779  
10. RTSSEDNLYLAVLR Phosphorylation: S4
55.41 IPI00398779  
11. RTSSEDNLYLAVLR Phosphorylation: T2
Phosphorylation: S3
Phosphorylation: S4
55.41 IPI00398779  
12. RTSSEDNLYLAVLR Phosphorylation: T2
Phosphorylation: S3
Phosphorylation: S4
45.87 IPI00398779  
13. RTSSEDNLYLAVLR Phosphorylation: S4
37.02 IPI00398779  
14. KRTSSEDNLYLAVLR Phosphorylation: S4
Phosphorylation: S5
26.91 IPI00398779  





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