HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00001
Experiment type Mass spectrometry
Short description Brain proteome analysis
Experimental description Brain tissue was homgenised and separated out using 2D gel electrophoresis. Protein spots differing between schizophrenia bipolar disorder and major depression, in comparison with controls, were identified using MALDI-TOF or LC-MS/MS
Principal Investigator's NameDr. Michael Dunn
TitleProfessor
E-mailmichael.dunn@ucd.ie
AddressUCD Conway Institute
University College Dubl
Belfield, Dublin 4
CountryIreland
Lab URLhttp://www.ucd.ie/conway
Data submitted byDr. Kyla Pennington
TitlePostdoctoral Researcher
E-mailkyla.pennington@ucd.ie
Published/Unpublished Published
Journal name
PubMed ID 16637010
Sample source
Tissue: Brain [eVOC:EV:0100164]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique None
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation Yes
Mass spectrometer
Instrument: 1. Q-Tof micro [PSI:1000188]
2. Voyager-DE PRO [PSI:1000203]
Vendor: 1. Waters [PSI:1000126]
2. ABI/SCIEX [PSI:1000121]
Ionization method 1. ESI [PSI:1000073]
2. MALDI [PSI:1000075]
Fragmentation method 1. CID [PSI:1000133]
Mass tolerance used for database searching (MS) 100 ppm
Mass tolerance used for database searching (MS/MS) 100 ppm
Database used for searching ProteinLynx
Search engine used Mascot
Download MS raw dataset  
Download processed files  



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