HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00002
Experiment type Mass spectrometry
Short description Plasma proteome analysis
Experimental description Plasma was depleted first with the top 12 abundance proteins and then the rest of proteins were separated with multi-lectin affinity column to have two pools (bound and unbound). Each pool was digested with trypsin with reduction and alkylation. The digested peptides were analyzed by LC-MS. This dataset represents bound pool.
Principal Investigator's NameDr. William Hancock
TitleProfessor
E-mailwi.hancock@neu.edu
AddressBradstreet Chair Barnett Institute
Department of Chemistry and Chemical Biology, Northeastern University 360 Huntington Avenue
Boston, MA 2115
CountryUSA
Data submitted byDr. Shiaw-Lin (Billy) Wu
TitleResearch Associate Professor
E-mailsi.wu@neu.edu
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue: Blood plasma [eVOC:EV:0100488]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique None
Protease used Trypsin
Is the sample from in gel No
Reduction and Alkylation Yes
Mass spectrometer
Instrument: LTQ [PSI:1000447]
Vendor: ThermoFinnigan [PSI:1000125]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 1000 ppm
Mass tolerance used for database searching (MS/MS) 1000 ppm
Database used for searching NCBI nr
Search engine used SEQUEST
Download MS raw dataset  
Download processed files  



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