HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00003
Experiment type Mass spectrometry
Short description B Cell proteome analysis
Experimental description Protein indentifications are made by replicate 2D-LC-MS/MS analysis of tryptic peptides from detergent solubilized B cell membrane proteins, as well as replicate LC-MS/MS analysis of single off-line strong cation exchange chromatography (SCX) fractions
Principal Investigator's NameDr. David Muddiman
TitleProfessor
E-maildavid_muddiman@ncsu.edu
AddressNorth Carolina State University
608E Cox Hall
Raleigh, NC 27695
CountryUSA
Lab URLhttp://www.ncsu.edu/chemistry/dcm/index.html
Published/Unpublished Published
Journal name
PubMed ID 15797518
Sample source
Tissue: B Cell [eVOC:EV:0200077]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique
Protease used Trypsin
Is the sample from in gel No
Reduction and Alkylation No
Mass spectrometer
Instrument: Q-Tof micro [PSI:1000188]
Vendor: Waters [PSI:1000126]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 0.5 Da
Mass tolerance used for database searching (MS/MS) 0.5 Da
Database used for searching RefSeq
Search engine used Mascot
Download MS raw dataset  
Download processed files  



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