Experimental Details - HuPA_00007
Experiment type Mass spectrometry
Short description Platelet subproteome analysis
Experimental description Commercially available kits were used for crude fractionation of cells into different compartments, such as membranes, cytosol, nucleus and cytoskeleton. Platelet proteome, activated and resting states, was characterized and compared.
Principal Investigator's NameDr. Gerard Cagney
TitleAssistant Professor
AddressConway Institute
University College Dublin
Belfield, Dublin 4
Data submitted byMathew Sullivan
TitleBioInformatics Group Leader
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue: Platelet [eVOC:EV:0200138]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation No
Mass spectrometer
Instrument: LCQ Classic [PSI:1000168]
Vendor: ThermoFinnigan [PSI:1000125]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 1.5 Da
Mass tolerance used for database searching (MS/MS) 0.5 Da
Database used for searching RefSeq
Search engine used SEQUEST
Download MS raw dataset  
Download processed files  

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