HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00008
Experiment type Mass spectrometry
Short description Serum proteome analysis
Experimental description Immunoglobulins were removed from serum using protein A/G, and the remaining proteins were digested with trypsin. Resulting peptides were separated by strong cation exchange chromatography into distinct fractions prior to analysis.
Principal Investigator's NameDr. Richard Smith
TitleLaboratory Fellow and Resource Director
E-maildick.smith@pnl.gov
AddressProteomics Research Resource for Integrative Biology
P.O. Box 999, K8-91
Richland, WA 99352
CountryUSA
Lab URLhttp://ncrr.pnl.gov
Data submitted bySamuel O Purvine
TitleSenior Research Scientist
E-mailsamuel.purvine@pnl.gov
Published/Unpublished Published
Journal name
PubMed ID 12543931
Sample source
Tissue: Serum [eVOC:EV:0100489]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique
Protease used Trypsin
Is the sample from in gel No
Reduction and Alkylation No
Mass spectrometer
Instrument: LCQ Deca XP Plus [PSI:1000169]
Vendor: ThermoFinnigan [PSI:1000125]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 3.0 Da
Mass tolerance used for database searching (MS/MS) 0.0 Da
Database used for searching RefSeq
Search engine used Mascot
Download MS raw dataset  
Download processed files  



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