HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00012
Experiment type Mass spectrometry
Short description Her2/neu tyrosine phosphoproteome anlaysis
Experimental description Three state SILAC experiment where tyrosine phosphorylation events in Her2/neu and downstream proteins were measured by anti-phosphotyrosine antibody pulldown followed by LC-MS/MS
Principal Investigator's NameDr. Philip Cole
TitleProfessor
E-mailpcole@jhmi.edu
AddressJohns Hopkins University
Hunterian 316, 725 N. Wolfe St.
Baltimore, MD 21205
CountryUSA
Published/Unpublished Published
Journal name
PubMed ID 16785428
Sample source
Tissue:
Cell line: NIH3T3
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique 13C6-arginine and 13C6,15N4-arginine (please note that this peptide does not contain Arg and therefore was not labelled with these isotopes)
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation Yes
Mass spectrometer
Instrument: QSTAR [PSI:1000190]
Vendor: ABI/SCIEX [PSI:1000121]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 0.3 Da
Mass tolerance used for database searching (MS/MS) 0.3 Da
Database used for searching RefSeq
Search engine used Mascot
Download MS raw dataset  
Download processed files  



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