HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00018
Experiment type Mass spectrometry
Short description Hair shaft proteome analysis
Experimental description Insoluble proteins from human hair shaft was extracted with extensive solubilization and reduced, alkylated, and digested. Digested peptide mixture was fractionated with off-link SCX before nanoLC-MS/MS.
Principal Investigator's NameDr. Robert H. Rice
TitleProfessor
E-mailrhrice@ucdavis.edu
AddressUniversity of California, Davis
One Shields Avenue
Davis, CA 95616
CountryUSA
Lab URLhttp://www.envtox.ucdavis.edu/Faculty_Personal_Web_Pages/rhrice_files/NewRice.htm
Published/Unpublished Published
Journal name
PubMed ID 16446289
Sample source
Tissue: Hair [eVOC:EV:0100157]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique None
Protease used Trypsin
Is the sample from in gel No
Reduction and Alkylation Reduction w/ 20mM DTE in 2%SDS, 50mM phsophate buffer and alkylation w/ 40mM IoAA
Mass spectrometer
Instrument: LCQ Deca XP Plus [PSI:1000169]
Vendor: Waters [PSI:1000126]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 2.5 Da
Mass tolerance used for database searching (MS/MS) 1 Da
Database used for searching IPI
Search engine used SEQUEST
Download MS raw dataset  
Download processed files  



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