Experimental Details - HuPA_00022
Experiment type Mass spectrometry
Short description Lung proteome analysis
Experimental description Bronchoalveolar lavage drawn from five healthy patients, supernatant and cellular fractions separated, supernatant fraction digested in solution with trypsin, analyzed by nanoUPLC-MS/MS in data dependent fashion
Principal Investigator's NameDr. Thomas Neubert
TitleAssociate Professor
AddressSkirball Institute for Biomolecular Medicine
New York University Medical Center, 540 First Ave., Lab 5-18
New York, NY 10016
Data submitted byDr. Helene Cardasis
TitlePostdoctoral Fellow
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue: Lung [eVOC:EV:0100042]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique None
Protease used Trypsin
Is the sample from in gel No
Reduction and Alkylation Yes
Mass spectrometer
Instrument: LTQ Orbitrap [PSI:1000449]
Vendor: ThermoFinnigan [PSI:1000125]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 0.3 Da
Mass tolerance used for database searching (MS/MS) 0.7 Da
Database used for searching NCBI
Search engine used Mascot
Download MS raw dataset  
Download processed files  

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