Experimental Details - HuPA_00026
Experiment type Mass spectrometry
Short description B Cell proteome analysis
Experimental description Proteins affected by anti-mIgM stimulation were identified using 2-DE-based proteomics. We investigated the proteome profiles of stimulated and nonstimulated Ramos B-cells at eight time points during 5 d.
Principal Investigator's NameDr. Mauno Vihinen
TitleProfessor of Bioinformatics
AddressInstitute of Medical Technology
FI-33014 University of Tampere
Biokatu, Tampere
Published/Unpublished Published
Journal name
PubMed ID 16947121
Sample source
Tissue: B Cell [eVOC:EV:0200077]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique SYPRO Ruby gel stain (Molecular Probes)
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation DTT and IAA
Mass spectrometer
Instrument: ultraFlex TOF/TOF [PSI:1000202]
Vendor: Bruker Daltonics [PSI:1000122]
Ionization method MALDI [PSI:1000075]
Fragmentation method PSD [PSI:1000135]
Mass tolerance used for database searching (MS) 50-150 ppm
Mass tolerance used for database searching (MS/MS) 80-150 ppm
Database used for searching MSDB
Search engine used Mascot
Download MS raw dataset  
Download processed files  

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