HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00028
Experiment type Mass spectrometry
Short description Histone modification analysis
Experimental description 293 HEK cells were collected and lysed and the soluble proteins cleared by ultracentrifugation. Histones were purifed from solution using rabbit polyclonal antibodies to histone H2A1.1 coupled to magnetic beads. The purified histones were isolated using 1-D SDS-PAGE and the histone bands excised. The histones were digested in an in-gel digestion and the peptides extracted from the gel slices. The isolated peptides were then run through a reverse-phase HPLC column into an ESI front-end to a QStar-XL.
Principal Investigator's NameDr. Alma Burlingame
TitleProfessor
E-mailalb@cgl.ucsf.edu
AddressMass Spectrometry Facility
521 Parnassus Ave., C-18, Mass Spectrometry Facility, University of California
San Francisco, CA 94143-0446
CountryUSA
Lab URLhttp://msf.ucsf.edu/
Data submitted byDr. Feixia Chu
TitlePostdoctoral Fellow
E-mailfchu@cgl.ucsf.edu
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue:
Cell line: 293 HEK
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique None
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation No
Mass spectrometer
Instrument: QSTAR [PSI:1000190]
Vendor: ABI/SCIEX [PSI:1000121]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 200 ppm
Mass tolerance used for database searching (MS/MS) 0.2 Da
Database used for searching SwissProt
Search engine used Mascot
Download MS raw dataset  
Download processed files  



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