HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00031
Experiment type Mass spectrometry
Short description Phosphoproteome analysis
Experimental description 100ug of total cell lysate was run on the SDS-PAGE and the whole lane was in-gel digested with trypsin. From the exracted peptide mixture, phosphopeptides were enriched with TiO2.
Principal Investigator's NameDr. Blagoy Blagoev
TitleAssistant Professor
E-mailbab@bmb.sdu.dk
AddressUniversity of Southern Denmark
Institute of Biochemistry and Molecular Biology, Campusvej 55,
Odense M, DK- 5230
CountryDenmark
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue: Mesenchymal cell [eVOC:EV:0200171]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique None
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation Yes
Mass spectrometer
Instrument: LTQ FT [PSI:1000448]
Vendor: ThermoFinnigan [PSI:1000125]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 10 ppm
Mass tolerance used for database searching (MS/MS) 0.6 Da
Database used for searching IPI
Search engine used Mascot
Download MS raw dataset  
Download processed files  



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