HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00033
Experiment type Mass spectrometry
Short description Brain proteome analysis
Experimental description Proteins were prepared from microdissected gray matter using 8 M urea, reduced, alkylated, digested and analyzed CITP-LC-MS/MS
Principal Investigator's NameDr. Brian Balgley
TitleDirector of Proteomics
E-mailbrian.balgley@calibrant.com
AddressCalibrant Biosystems
910 Clopper Rd. Ste 220N
Gaithersburg, MD 20878
CountryUSA
Data submitted byDr. Wejie Wang
TitleScientist
E-mailweijie.wang@calibrant.com
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue: Brain [eVOC:EV:0100164]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique None
Protease used Trypsin
Is the sample from in gel No
Reduction and Alkylation Yes
Mass spectrometer
Instrument: LTQ [PSI:1000447]
Vendor: ThermoFinnigan [PSI:1000125]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 1.5 Da
Mass tolerance used for database searching (MS/MS) 0.4 Da
Database used for searching SwissProt
Search engine used OMSSA
Download MS raw dataset  
Download processed files  



Please send any questions or comments about Human Proteinpedia to help
This is a joint project between:
   and