Experimental Details - HuPA_00036
Experiment type Mass spectrometry
Short description Ovary proteome analysis
Experimental description Proteins were extracted from microdissected tissue from normal ovary using 8 M urea, reduced, alkylated, digested and analyzed CIEF-LC-MS/MS
Principal Investigator's NameDr. Brian Balgley
TitleDirector of Proteomics
AddressCalibrant Biosystems
910 Clopper Rd. Ste 220N
Gaithersburg, MD 20878
Data submitted byDr. Wejie Wang
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue: Ovary [eVOC:EV:0100111]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique None
Protease used Trypsin
Is the sample from in gel No
Reduction and Alkylation Yes
Mass spectrometer
Instrument: LTQ [PSI:1000447]
Vendor: ThermoFinnigan [PSI:1000125]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 1.5 Da
Mass tolerance used for database searching (MS/MS) 0.4 Da
Database used for searching SwissProt
Search engine used OMSSA
Download MS raw dataset  
Download processed files  

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