HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00037
Experiment type Mass spectrometry
Short description Adipose derived stem cell analysis
Experimental description Primary human adipose derived stem cells were cultured in the undifferentiated and adipocyte differentiated states. Secreted proteins from each were obtained in serum free conditioned medium, separated by 2D PAGE, and analyzed by MS.
Principal Investigator's NameDr. Jeffrey Gimble
TitleProfessor
E-mailGimblejm@pbrc.edu
AddressPennington Biomedical Research Center
6400 Perkins Road
Baton Rouge, LA 70808
CountryUSA
Data submitted byDr. Indu Kheterpal
TitleAssistant Professor
E-mailindu.kheterpal@pbrc.edu
Published/Unpublished Published
Journal name
PubMed ID 17018519
Sample source
Tissue: Stem cell [eVOC:EV:0200152]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique None
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation Yes
Mass spectrometer
Instrument: Q-Tof micro [PSI:1000188]
Vendor: Waters [PSI:1000126]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 0.2 Da
Mass tolerance used for database searching (MS/MS) 0.2 Da
Database used for searching RefSeq
Search engine used Mascot
Download MS raw dataset  
Download processed files  



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