Experimental Details - HuPA_00038
Experiment type Mass spectrometry
Short description Bone marrow stromal cell proteome analysis
Experimental description Bone marrow stromal cells from normal individuals and leukemia patients were culture-expanded; total proteins were extracted; they were reduced, and alkylated with acrylamide; digested with trypsin; and labeled with iTRAQ reagents, followed by protein identification and relative quantification using 2-D LC MS/MS.
Principal Investigator's NameDr. Beerelli Seshi
TitleHead, Hematopathology
AddressHarbor-UCLA Medical Center and
Los Angeles Biomedical Research Institute
Torrance, CA 90502
Published/Unpublished Published
Journal name
PubMed ID 16947122
Sample source
Tissue: Bone marrow [eVOC:EV:0100046]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique iTRAQ used for labeling
Protease used Trypsin
Is the sample from in gel No
Reduction and Alkylation Yes
Mass spectrometer
Instrument: QSTAR [PSI:1000190]
Vendor: ABI/SCIEX [PSI:1000121]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 0.15 Da
Mass tolerance used for database searching (MS/MS) 0.1 Da
Database used for searching CDS, SwissProt and IPI Human
Search engine used ProQuant software v1.1
Download MS raw dataset  
Download processed files  

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