HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00038
Experiment type Mass spectrometry
Short description Bone marrow stromal cell proteome analysis
Experimental description Bone marrow stromal cells from normal individuals and leukemia patients were culture-expanded; total proteins were extracted; they were reduced, and alkylated with acrylamide; digested with trypsin; and labeled with iTRAQ reagents, followed by protein identification and relative quantification using 2-D LC MS/MS.
Principal Investigator's NameDr. Beerelli Seshi
TitleHead, Hematopathology
E-mailbseshi@labiomed.org
AddressHarbor-UCLA Medical Center and
Los Angeles Biomedical Research Institute
Torrance, CA 90502
CountryUSA
Lab URLhttp://www.labiomed.org/stromalcell/index.htm
Published/Unpublished Published
Journal name
PubMed ID 16947122
Sample source
Tissue: Bone marrow [eVOC:EV:0100046]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique iTRAQ used for labeling
Protease used Trypsin
Is the sample from in gel No
Reduction and Alkylation Yes
Mass spectrometer
Instrument: QSTAR [PSI:1000190]
Vendor: ABI/SCIEX [PSI:1000121]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 0.15 Da
Mass tolerance used for database searching (MS/MS) 0.1 Da
Database used for searching CDS, SwissProt and IPI Human
Search engine used ProQuant software v1.1
Download MS raw dataset  
Download processed files  



Please send any questions or comments about Human Proteinpedia to help
This is a joint project between:
   and