Experimental Details - HuPA_00041
Experiment type Mass spectrometry
Short description Saliva proteome analysis
Experimental description Whole and parotid salivas were donated by adult healthy subjects. Saliva proteins were concentratred by TCA/acetone and separated on 2D electrophoretic gels. Excised spots were identified after tryptic digestion.
Principal Investigator's NameDr. Eric Dransfield
TitlePrincipal Investigator
WCFS, Diedenweg 20
Wageningen, AN 6700
CountryThe Netherlands
Data submitted byMartine Morzel
TitleResearch scientist
Published/Unpublished Published
Journal name
PubMed ID 16944961
Sample source
Tissue: Saliva [eVOC:EV:0100515]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique None
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation Yes
Mass spectrometer
Instrument: LCQ Deca XP Plus [PSI:1000169]
Vendor: Waters [PSI:1000126]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 1.4 Da
Mass tolerance used for database searching (MS/MS) 0.8 Da
Database used for searching Swiss-Prot/Trembl
Search engine used Bioworks 3.1
Download MS raw dataset  
Download processed files  

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