HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00041
Experiment type Mass spectrometry
Short description Saliva proteome analysis
Experimental description Whole and parotid salivas were donated by adult healthy subjects. Saliva proteins were concentratred by TCA/acetone and separated on 2D electrophoretic gels. Excised spots were identified after tryptic digestion.
Principal Investigator's NameDr. Eric Dransfield
TitlePrincipal Investigator
E-maileric.dransfield@wur.nl
AddressINRA
WCFS, Diedenweg 20
Wageningen, AN 6700
CountryThe Netherlands
Data submitted byMartine Morzel
TitleResearch scientist
E-mailMartine.Morzel@dijon.inra.fr
Published/Unpublished Published
Journal name
PubMed ID 16944961
Sample source
Tissue: Saliva [eVOC:EV:0100515]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique None
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation Yes
Mass spectrometer
Instrument: LCQ Deca XP Plus [PSI:1000169]
Vendor: Waters [PSI:1000126]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 1.4 Da
Mass tolerance used for database searching (MS/MS) 0.8 Da
Database used for searching Swiss-Prot/Trembl
Search engine used Bioworks 3.1
Download MS raw dataset  
Download processed files  



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