Experimental Details - HuPA_00044
Experiment type Mass spectrometry
Short description Liver normal and tumor tissue analysis
Experimental description Liver tissue was homogenized and proteins were digested with trypsin without reduction or alkylation. The extracted peptides from nontumor and tumor parts were labeled with iTRAQ reagent with 114, 115 and 116, 117 (114 and 115 for normal liver samples and 116 and 117 from hepatocellular carcinoma).
Principal Investigator's NameDr. Akhilesh Pandey
AddressJohns Hopkins University
527 BRB, 733 N.Broadway
Baltimore, MD 21205
Data submitted byDr. Raghothama Chaerkady
TitlePost Doctoral Fellow
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue: Liver [eVOC:EV:0100089]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique iTRAQ
Protease used Trypsin
Is the sample from in gel No
Reduction and Alkylation No
Mass spectrometer
Instrument: QSTAR [PSI:1000190]
Vendor: ABI/SCIEX [PSI:1000121]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 100 ppm
Mass tolerance used for database searching (MS/MS) 100 ppm
Database used for searching RefSeq
Search engine used Mascot
Download MS raw dataset  
Download processed files  

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