| Experimental Details - HuPA_00044 |
| Experiment type |
Mass spectrometry |
| Short description |
Liver normal and tumor tissue analysis |
| Experimental description |
Liver tissue was homogenized and proteins were digested with trypsin without reduction or alkylation. The extracted peptides from nontumor and tumor parts were labeled with iTRAQ reagent with 114, 115 and 116, 117 (114 and 115 for normal liver samples and 116 and 117 from hepatocellular carcinoma). |
| Principal Investigator's Name | Dr. Akhilesh Pandey |
| Title | Professor |
| E-mail | pandey@jhmi.edu |
| Address | Johns Hopkins University
527 BRB, 733 N.Broadway
Baltimore, MD 21205 |
| Country | USA |
| Lab URL | http://pandeylab.igm.jhmi.edu |
| Data submitted by | Dr. Raghothama Chaerkady |
| Title | Post Doctoral Fellow |
| E-mail | raghothama@jhmi.edu |
| Published/Unpublished |
Unpublished
|
| Journal name |
Not applicable |
| PubMed ID |
Not applicable |
| Sample source |
|
| Source organism |
Homo sapiens [Taxonomy:9606] |
| Labeling technique |
iTRAQ |
| Protease used |
Trypsin |
| Is the sample from in gel |
No |
| Reduction and Alkylation |
No |
| Mass spectrometer |
|
| Ionization method |
ESI [PSI:1000073] |
| Fragmentation method |
CID [PSI:1000133] |
| Mass tolerance used for database searching (MS) |
100 ppm |
| Mass tolerance used for database searching (MS/MS) |
100 ppm |
| Database used for searching |
RefSeq |
| Search engine used |
Mascot |
| Download MS raw dataset |
|
| Download processed files |
|
