HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00048
Experiment type Mass spectrometry
Short description Platelet acetylation analysis
Experimental description Strong cation exchange separation followed by COFRADIC peptide selection by methionine oxidation, which induces a chromatographic shift on a diagonal RP-HPLC system.
Principal Investigator's NameDr. Kris Gevaert
TitleProfessor
E-mailkris.gevaert@ugent.be
AddressDepartment of Medical Protein Research, Proteome Analysis and Bioinformatics Unit
Flanders Interuniversity Institute for Biotechnology
A. Baertsoenkaai 3, Gent
CountryBelgium
Lab URLhttp://www.proteomics.be/
Published/Unpublished Published
Journal name
PubMed ID 16038019
Sample source
Tissue: Platelet [eVOC:EV:0200138]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique
Protease used Trypsin
Is the sample from in gel
Reduction and Alkylation
Mass spectrometer
Instrument: Q-Tof micro [PSI:1000188]
Vendor: Waters [PSI:1000126]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 0.3 Da
Mass tolerance used for database searching (MS/MS) 0.3 Da
Database used for searching IPI
Search engine used Mascot
Download MS raw dataset  
Download processed files  



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