Experimental Details - HuPA_00053
Experiment type Mass spectrometry
Short description Urinary proteome analysis
Experimental description Proteins in lyophilized urine were resolved with SDS-PAGE, visualized with Coomassie Brilliant Blue R-250 stain, and identified by Q-TOF MS/MS
Principal Investigator's NameDr. Visith Thongboonkerd
TitlePrincipal Investigator
AddressSiriraj Proteomics Facility, Faculty of Medicine Siriraj Hospital, Mahidol University
Medical Molecular Biology Unit, Office for Research and Development, 12th Floor Adulyadej Vikrom Building, Siriraj Hospital, 2 Prannok Road, Bangkoknoi
Bangkok, - 10700
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue: Urine [eVOC:EV:0000002]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique None
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation Yes
Mass spectrometer
Instrument: Q-Tof Ultima [PSI:1000189]
Vendor: Waters [PSI:1000126]
Ionization method MALDI [PSI:1000075]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 50 ppm
Mass tolerance used for database searching (MS/MS) 0.6 Da
Database used for searching RefSeq
Search engine used Mascot
Download MS raw dataset  
Download processed files  

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