HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00053
Experiment type Mass spectrometry
Short description Urinary proteome analysis
Experimental description Proteins in lyophilized urine were resolved with SDS-PAGE, visualized with Coomassie Brilliant Blue R-250 stain, and identified by Q-TOF MS/MS
Principal Investigator's NameDr. Visith Thongboonkerd
TitlePrincipal Investigator
E-mailthongboonkerd@dr.com
AddressSiriraj Proteomics Facility, Faculty of Medicine Siriraj Hospital, Mahidol University
Medical Molecular Biology Unit, Office for Research and Development, 12th Floor Adulyadej Vikrom Building, Siriraj Hospital, 2 Prannok Road, Bangkoknoi
Bangkok, - 10700
CountryThailand
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue: Urine [eVOC:EV:0000002]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique None
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation Yes
Mass spectrometer
Instrument: Q-Tof Ultima [PSI:1000189]
Vendor: Waters [PSI:1000126]
Ionization method MALDI [PSI:1000075]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 50 ppm
Mass tolerance used for database searching (MS/MS) 0.6 Da
Database used for searching RefSeq
Search engine used Mascot
Download MS raw dataset  
Download processed files  



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