Experimental Details - HuPA_00054
Experiment type Mass spectrometry
Short description Brain proteome analysis
Experimental description Brain tissue was grown as xenografts in mice. Tissue was harvested, homogenized, trypsin digested as per ABI iTRAQ protocol. Four samples were labeled and pooled. All four were human brain tissue.
Principal Investigator's NameDr. K.W. Michael Siu
TitleProfessor of Chemistry and Director CRMS
AddressCentre for Research in Mass Spectrometry
Centre for Research in Mass Spectrometry, Chemistry Department 4700 Keele St.
Toronto, ON M3J 1P3
Data submitted byLeroi V. DeSouza
TitleManager - Proteomics facility
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue: Brain [eVOC:EV:0100164]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique iTRAQ
Protease used Trypsin
Is the sample from in gel No
Reduction and Alkylation Reduction with TCEP and alkylation with MMTS
Mass spectrometer
Instrument: QSTAR [PSI:1000190]
Vendor: ABI/SCIEX [PSI:1000121]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS)
Mass tolerance used for database searching (MS/MS)
Database used for searching ABI supplied Celera Database
Search engine used ProteinPilot
Download MS raw dataset  
Download processed files  

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