Experimental Details - HuPA_00056
Experiment type Mass spectrometry
Short description Ubiquitination analysis
Experimental description Cell lysates immunoprecipitated with an anti-Ubiquitin antibody. Ubiquitinated proteins digested with trypsin and analyzed by LC-MS/MS
Principal Investigator's NameDr. Daniel Figeys
AddressProteomics Resource Center
Ottawa Institute of Systems Biology, University of Ottawa
Ottawa, ON K1H 8M5
Data submitted byJulian Vasilescu
TitleFacility Manager
Published/Unpublished Published
Journal name
PubMed ID 17203973
Sample source
Cell line: H1299
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique None
Protease used Trypsin
Is the sample from in gel No
Reduction and Alkylation Yes
Mass spectrometer
Instrument: QSTAR [PSI:1000190]
Vendor: ABI/SCIEX [PSI:1000121]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 100 ppm
Mass tolerance used for database searching (MS/MS) 0.2 Da
Database used for searching NCBInr
Search engine used Mascot
Download MS raw dataset  
Download processed files  

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