Experimental Details - HuPA_00058
Experiment type Mass spectrometry
Short description Sperm proteome analysis
Experimental description Sperm was lysed and runned in 2D gel. Spots were picked and digested with trypsin with reduction and alkylation.
Principal Investigator's NameDr. Rafael Oliva
AddressUniversitat de Barcelona and Hospital Clinic
Casanova 143
Data submitted byJuan Martinez-Heredia
TitlePh.D. Student
Published/Unpublished Published
Journal name
PubMed ID 16819732
Sample source
Tissue: Spermatozoa [eVOC:EV:0200045]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique None
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation Reduction: DTT; Alkylation: Iodoacetamide
Mass spectrometer
Instrument: Voyager-DE PRO [PSI:1000203]
Vendor: ABI/SCIEX [PSI:1000121]
Ionization method MALDI [PSI:1000075]
Fragmentation method
Mass tolerance used for database searching (MS) 75 ppm
Mass tolerance used for database searching (MS/MS)
Database used for searching SwissProt
Search engine used Protein Prospector, MSFit
Download MS raw dataset  
Download processed files  

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