HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00135
Experiment type Mass spectrometry
Short description Pancreatic juice analysis
Experimental description This sample is a pancreatic juice specimen from a patient with chronic pancreatitis. The pancreatic juice sample was initially resolved on a 1D gradient gel (4-12%), bands were excised and digested by trypsin. The extracted peptides were treated with PNGaseF in 18O-labeled water (this converts asparagines to aspartic acid with a total mass increase of 3 Da due to 18O incorporation) and analyzed by LC-MS/MS.
Principal Investigator's NameDr. Akhilesh Pandey
TitleProfessor
E-mailpandey@jhmi.edu
AddressJohns Hopkins University
527 BRB, 733 N.Broadway
Baltimore, MD 21205
CountryUSA
Lab URLhttp://pandeylab.igm.jhmi.edu
Data submitted bySuresh Mathivanan
TitlePh.D. Student
E-mailsuresh@jhmi.edu
Published/Unpublished Published
Journal name
PubMed ID 15473694
Sample source
Tissue: Pancreatic juice [eVOC:EV:0100511]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique 18O-labeling
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation DTT and iodoacetamide
Mass spectrometer
Instrument: QSTAR [PSI:1000190]
Vendor: ABI/SCIEX [PSI:1000121]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 0.3 Da
Mass tolerance used for database searching (MS/MS) 0.2 Da
Database used for searching RefSeq
Search engine used Mascot
Download MS raw dataset  
Download processed files  



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