| Experimental Details - HuPA_00136 |
| Experiment type |
Mass spectrometry |
| Short description |
Pancreatic juice analysis |
| Experimental description |
This sample is a pancreatic juice specimen from a patient with chronic pancreatitis. The pancreatic juice sample was initially resolved on a 1D gradient gel (4-12%), bands were excised and digested by trypsin. The extracted peptides were treated with PNGaseF in 18O-labeled water (this converts asparagines to aspartic acid with a total mass increase of 3 Da due to 18O incorporation) and analyzed by LC-MS/MS. |
| Principal Investigator's Name | Dr. Akhilesh Pandey |
| Title | Professor |
| E-mail | pandey@jhmi.edu |
| Address | Johns Hopkins University
527 BRB, 733 N.Broadway
Baltimore, MD 21205 |
| Country | USA |
| Lab URL | http://pandeylab.igm.jhmi.edu |
| Data submitted by | Suresh Mathivanan |
| Title | Ph.D. Student |
| E-mail | suresh@jhmi.edu |
| Published/Unpublished |
Published
|
| Journal name |
|
| PubMed ID |
15473694
|
| Sample source |
|
| Source organism |
Homo sapiens [Taxonomy:9606] |
| Labeling technique |
18O-labeling |
| Protease used |
Trypsin |
| Is the sample from in gel |
Yes |
| Reduction and Alkylation |
DTT and iodoacetamide |
| Mass spectrometer |
|
| Ionization method |
ESI [PSI:1000073] |
| Fragmentation method |
CID [PSI:1000133] |
| Mass tolerance used for database searching (MS) |
0.3 Da |
| Mass tolerance used for database searching (MS/MS) |
0.2 Da |
| Database used for searching |
RefSeq |
| Search engine used |
Mascot |
| Download MS raw dataset |
|
| Download processed files |
|
