HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00138
Experiment type Mass spectrometry
Short description Cerebrospinal fluid analysis
Experimental description Pooled post-mortem CSF samples were analyzed using a protocol that combined immunoaffinity depletion of abundant CSF proteins, off-gel electrophoresis, SDS-PAGE, trypsin digestion without reduction or alkylation and protein identification by LC-MS/MS
Principal Investigator's NameDr. Jean-Charles Sanchez
TitleAssistant Professor
E-mailJean-Charles.Sanchez@medecine.unige.ch
AddressBiomedical Proteomics Research Group (BPRG)
Biomedical Proteomics Research Group (BPRG), Department of Structural Biology and Bioinformatics, University Medical Centre (CMU), University of Geneva, 1 rue Michel Servet 1211
Geneva, 4
CountrySwitzerland
Lab URLhttp://www.expasy.org/bprg/
Data submitted byAlexandre Hainard
TitleScientific collaborator
E-mailalexandre.hainard@medecine.unige.ch
Published/Unpublished Published
Journal name
PubMed ID 16823975
Sample source
Tissue: Cerebrospinal fluid [eVOC:EV:0100311]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique None
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation No
Mass spectrometer
Instrument: LCQ Deca XP Plus [PSI:1000169]
Vendor: ThermoFinnigan [PSI:1000125]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 2.0 Da
Mass tolerance used for database searching (MS/MS) 1.0 Da
Database used for searching UniProtSP
Search engine used Mascot
Download MS raw dataset  
Download processed files  



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