Experimental Details - HuPA_00150
Experiment type Mass spectrometry
Short description Jurkat cells analysis
Experimental description Combined fractional diagonal chromatography (COFRADIC) sorting of protein amino-terminal peptides coupled to oxygen-16 or oxygen-18 differential labeling was employed.
Principal Investigator's NameDr. Kris Gevaert
AddressDepartment of Medical Protein Research, Proteome Analysis and Bioinformatics Unit
Flanders Interuniversity Institute for Biotechnology
A. Baertsoenkaai 3, Gent
Published/Unpublished Published
Journal name
PubMed ID 16179924
Sample source
Cell line: Jurkat
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique O18 labelling
Protease used Trypsin
Is the sample from in gel No
Reduction and Alkylation Yes
Mass spectrometer
Instrument: Q-Tof micro [PSI:1000188]
Vendor: Waters [PSI:1000126]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 0.3 Da
Mass tolerance used for database searching (MS/MS) 0.3 Da
Database used for searching SwissProt & NCBI nr
Search engine used Mascot
Download MS raw dataset  
Download processed files  

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