HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00152
Experiment type Co-immunoprecipitation based protein-protein interaction
Short description Co-immunoprecipitation based protein-protein interaction analysis
Experimental description Enhanced green fluorescent protein (EGFP) fusion protein containing residues 144 to 847 of Vav-3 was expressed ectopically in Cos-1 cells. Transfected cells were made quiescent and then stimulated for 0, 5 and 10 min with EGF. Proteins were then immunoprecipitated with anti-GFP antibodies and immunoblotted with anti-PTyr antibodies and anti-Shc antibodies.
Principal Investigator's NameDr. Xose Bustelo
TitleProfessor
E-mailxbustelo@usal.es
AddressCenter for Cancer Research
Campus Miguel de Unamuno
Salamanca, Spain 37007
CountrySpain
Lab URLhttp://www.cicancer.org
Data submitted byDr. Nieves Ibarrola
TitlePh.D.
E-mailnibarrola@usal.es
Published/Unpublished Published
Journal name
PubMed ID 10523675
Sample source
Tissue: COS-1
Cell line:
Source organism Homo sapiens [Taxonomy:9606]



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