Experimental Details - HuPA_00361
Experiment type Mass spectrometry
Short description Ovarian tumor proteome analysis
Experimental description DIGE experiment. Picking gels were stained with ruthenium bathrenolate. The spot picking, digestion and extraction were carried out using a spot handling workstation (ETTAN Spot handling workstation, GE Healthcare) and a standard protocol provided by GE Healthcare.
Principal Investigator's NameDr. Peter James
AddressProtein Technology
Lund University, BMC D13
Lund, 22184
Data submitted byDr. Fredrik Levander
TitleAssistant Professor
Published/Unpublished Published
Journal name
PubMed ID 17315909
Sample source
Disease tissue: Ovarian tumor
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique DIGE
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation Yes
Mass spectrometer
Instrument: Q-Tof Ultima [PSI:1000189]
Vendor: Waters [PSI:1000126]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 0.1 Da
Mass tolerance used for database searching (MS/MS) 0.1 Da
Database used for searching IPI_Human 2.38
Search engine used Mascot
Download MS raw dataset  
Download processed files  

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