HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00379
Experiment type Mass spectrometry
Short description HeLa cell fractionization
Experimental description A HeLa cell protein extract was digested with trypsin, separated first using offgel electrophoresis into 23 fractions, and each fraction was ran using HPLC Chip-LC/MS/MS
Principal Investigator's NameDr. David Horn
TitleScientist
E-maildavid_horn@agilent.com
AddressAgilent Technologies
5301 Stevens Creek Blvd
Santa Clara, CA 95052
CountryUSA
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue:
Cell line: HeLa
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique None
Protease used Trypsin
Is the sample from in gel No
Reduction and Alkylation IAA
Mass spectrometer
Instrument: Agilent 6510 Q-Tof
Vendor: Agilent
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 10 ppm
Mass tolerance used for database searching (MS/MS) 40 ppm
Database used for searching IPI Human version 3.28
Search engine used Spectrum Mill
Download MS raw dataset  
Download processed files  



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