HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00380
Experiment type Mass spectrometry
Short description Identification of NPM-ALK interacting proteins by tandem mass spectrometry
Experimental description Immunoprecipitation (antibody IP) enrichment for NPM-ALK and its interacting proteins. IP enrichme XP PAGE and analyzed by MS/MS. Any proteins identified in this IgG control are not candidates (subtracted) for NPM-ALK protein-protein interactions.
Principal Investigator's NameDr. Kojo Elenitoba-Johnson
TitleAssociate Professor
E-mailkojoelen@med.umich.edu
AddressUniversity of Michigan Medical School
109 Zina Pitcher Place BSRB 4061
Ann Arbor, Michigan 48109
CountryUSA
Data submitted byDr. Megan S Lim
TitleAssociate Professor
E-mailmeganlim@umich.edu
Published/Unpublished Published
Journal name
PubMed ID 14968112
Sample source
Tissue:
Cell line: Karpas 299
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique None
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation Yes
Mass spectrometer
Instrument: LCQ Deca XP
Vendor: ThermoFinnigan
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 1.8 amu
Mass tolerance used for database searching (MS/MS) 2.0 amu
Database used for searching NCBI nr
Search engine used Bioworks 3.1 (SEQUEST)
Download MS raw dataset  
Download processed files  



Please send any questions or comments about Human Proteinpedia to help
This is a joint project between:
   and