HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00383
Experiment type Mass spectrometry
Short description Human erythroleukemia K562 analysis
Experimental description Improving reproducibility and sensitivity in identifying human proteins by shotgun proteomics.
Principal Investigator's NameDr. Natalie G Ahn
TitleAssociate Professor
E-mailNatalie.Ahn@colorado.edu
AddressUniversity of Colorado
Cristol Chemistry 200A
Boulder, CO 80309
CountryUSA
Published/Unpublished Published
Journal name
PubMed ID 15228325
Sample source
Tissue:
Cell line: K562
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique No
Protease used Trypsin
Is the sample from in gel
Reduction and Alkylation Yes
Mass spectrometer
Instrument: LCQ Classic [PSI:1000168]
Vendor: ThermoFinnigan [PSI:1000125]
Ionization method ESI [PSI:1000073]
Fragmentation method CID [PSI:1000133]
Mass tolerance used for database searching (MS) 2.5 Da
Mass tolerance used for database searching (MS/MS) 1.0 Da
Database used for searching IPI
Search engine used SEQUEST
Download MS raw dataset  
Download processed files  



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