HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00660
Experiment type Mass spectrometry
Short description Skeletal muscle tissue proteome analysis
Experimental description Muscle tissue was homogenized and proteins were separated by 2-DE. Excised spots were digested in situ with trypsin. The extracted peptides were analyzed by mass spectrometry.
Principal Investigator's NameDr. Cecilia Gelfi
TitleAssociate Professor
E-mailcecilia.gelfi@unimi.it
AddressUniversity of Milan
via F.lli Cervi 93
Segrate (MI), Italy 20090
CountryItaly
Data submitted byMichele Vasso
TitlePh.D. student
E-mailmichele.vasso@unimi.it
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue: Skeletal muscle [eVOC:EV:0100377]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique -
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation Dithiotreitol and Iodoacetamide
Mass spectrometer
Instrument: HCT Ultra PTM Discovery
Vendor: Bruker Daltonics
Ionization method ESI
Fragmentation method CID
Mass tolerance used for database searching (MS) 0.3 Da
Mass tolerance used for database searching (MS/MS) 0.3 Da
Database used for searching NCBI
Search engine used Mascot
Download MS raw dataset  
Download processed files  



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