Experimental Details - HuPA_00660
Experiment type Mass spectrometry
Short description Skeletal muscle tissue proteome analysis
Experimental description Muscle tissue was homogenized and proteins were separated by 2-DE. Excised spots were digested in situ with trypsin. The extracted peptides were analyzed by mass spectrometry.
Principal Investigator's NameDr. Cecilia Gelfi
TitleAssociate Professor
AddressUniversity of Milan
via F.lli Cervi 93
Segrate (MI), Italy 20090
Data submitted byMichele Vasso
TitlePh.D. student
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue: Skeletal muscle [eVOC:EV:0100377]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique -
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation Dithiotreitol and Iodoacetamide
Mass spectrometer
Instrument: HCT Ultra PTM Discovery
Vendor: Bruker Daltonics
Ionization method ESI
Fragmentation method CID
Mass tolerance used for database searching (MS) 0.3 Da
Mass tolerance used for database searching (MS/MS) 0.3 Da
Database used for searching NCBI
Search engine used Mascot
Download MS raw dataset  
Download processed files  

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