HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00663
Experiment type Mass spectrometry
Short description An Evaluation of several MS/MS search Algorithms for analysis of spectra derived from ETD experiments
Experimental description -
Principal Investigator's NameDr. Akhilesh Pandey
TitleProfessor
E-mailpandey@jhmi.edu
AddressInstitute of Bioinformatics
Institute of Bioinformatics
Bangalore, KA 560066
CountryINDIA
Data submitted byKumaran Kandasamy
TitlePh.D. student
E-mailkumaran@ibioinformatics.org
Published/Unpublished Published
Journal name
PubMed ID 19639959
Sample source
Tissue:
Cell line: UPS1 (SIGMA)
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique -
Protease used Trypsin
Is the sample from in gel No
Reduction and Alkylation DTT and iodoacetamide
Mass spectrometer
Instrument: Ion trap (Agilent)
Vendor: Agilent Technologies
Ionization method ESI
Fragmentation method ETD
Mass tolerance used for database searching (MS) 2.5 Da
Mass tolerance used for database searching (MS/MS) 0.7 Da
Database used for searching NCBI RefSeq (March 5, 2007)
Search engine used MASCOT; OMSSA; Spectrum Mill; X! Tandem
Download MS raw dataset  
Download processed files  



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