HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00670
Experiment type Mass spectrometry
Short description Urine ultrafiltrate analysis
Experimental description Urine was ultrafiltrated (cut-off: 30,000 Da) and ultrafiltrate was desalted with PD10 column. Peptides were sequenced without prior digestion
Principal Investigator's NameProf. Harald Mischak
TitleProfessor
E-mailmischak@mosaiques-diagnostics.com
AddressMosaiques & diagnostics GmbH
Director of mosaiques & diagnostics GmbH, Mellendorfer Str. 7-9
Hannover, 30625
CountryGermany
Lab URLhttp://www.mosaiques-diagnostics.com
Data submitted byDr. Petra Zürbig
TitleSenior Scientist
E-mailzuerbig@mosaiques-diagnostics.com
Published/Unpublished Published
Journal name
PubMed ID 20616184
Sample source
Tissue: Urine [eVOC:EV:0000002]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique No
Protease used No
Is the sample from in gel No
Reduction and Alkylation No
Mass spectrometer
Instrument: Orbitrap (Thermo); MicroTOFQ (Bruker); LTQ (Thermo); Ultraflex (Bruker)
Vendor: Thermo; Bruker
Ionization method ESI [PSI:1000073], MALDI
Fragmentation method CID [PSI:1000133], ETD
Mass tolerance used for database searching (MS) 0.5 Da
Mass tolerance used for database searching (MS/MS) 0.7 Da
Database used for searching Swissprot
Search engine used Mascot
Download MS raw dataset  
Download processed files  



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