Experimental Details - HuPA_00672
Experiment type Mass spectrometry
Short description Profiling of urinary proteome
Experimental description In this study we have carried out comprehensive analysis of human urinary proteome from healthy individuals. Gel based fractionation approach was employed followed by in-depth analysis using high resolution Fourier transform mass spectrometer. Urinary glycoproteins were also analyzed by employing lectin affinity based selective enrichment strategy. A total of 1,823 proteins were identified in our study, of which, 671 proteins have not been reported previously in human urine. We identified 617 proteins that were selectively enriched by lectin affinity enrichment which could be potentially used as biomarkers. This dataset will serve as a comprehensive reference list for future studies aimed at identification and characterization of urinary markers for various diseases.
Principal Investigator's NameAkhilesh Pandey
AddressInstitute of Bioinformatics
Johns Hopkins University
Baltimore, Maryland 21205
Data submitted byArivusudar Marimuthu
TitlePh.D. student
Published/Unpublished Published
Journal name
PubMed ID 21500864
Sample source
Tissue: Urine [eVOC:EV:0000002]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique None
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation Yes
Mass spectrometer
Instrument: LTQ-Orbitrap Velos
Vendor: Thermo Scientific
Ionization method ESI [PSI:1000073]
Fragmentation method HCD
Mass tolerance used for database searching (MS) 0.1 Da
Mass tolerance used for database searching (MS/MS) 20 PPM
Database used for searching RefSeq Release 40 - Human
Search engine used Mascot and Sequest
Download MS raw dataset  
Download processed files  

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