Experimental Details - HuPA_00682
Experiment type Mass spectrometry
Short description Qualitative proteomic analysis of human normal vitreous humor
Experimental description We employed multiple fractionation techniques namely, SDS-PAGE followed by in-gel digestion, in solution digestion followed by SCX chromatography and OFFGEL. we performed 70 LC-MS/MS runs for in-gel, SCX and OFFGEL fractions. The resulting mass spectral data was searched against the NCBI RefSeq-45 human protein database using three different search algorithms - Mascot, SEQUEST and X! Tandem
Principal Investigator's NameDr. Akhilesh Pandey
AddressInstitute of Bioinformatics
7th floor, Discoverer building
Bangalore, Karnataka 560066
Data submitted byDr. Renu Goel
TitleFaculty Scientist
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue: Vitreous humor [eVOC:EV:3000433]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique No
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation Yes
Mass spectrometer
Instrument: LTQ-Orbitrap Velos
Vendor: Thermo Fisher
Ionization method Nanospray
Fragmentation method HCD
Mass tolerance used for database searching (MS) 20 ppm
Mass tolerance used for database searching (MS/MS) 0.1 Da
Database used for searching Refseq45
Search engine used Sequest, Mascot, X!tandem
Download MS raw dataset  
Download processed files  

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