HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00691
Experiment type Mass spectrometry
Short description Analysis of urine proteome via a combination of multidimensional approaches
Experimental description Analysis of urine proteome via a combination of multidimensional approaches.Urine proteins were separated by three different methods-directly on SDS_PAGE, fractionated by liquid chromatography followed by SDS-PAGE and fractionation by preparative electrophoresis followed by SDS-PAGE. All gel bands were excised, trypsinized and analyzed on LC-MS/MS
Principal Investigator's NameAntonia Vlahou
TitleProffesor
E-mailvlahoua@bioacademy.gr
AddressBiomedical Research Foundation
Soranou Efesiou 4
Athens, Attiki 115 27
CountryGreece
Data submitted byPanagiotis Zerefos
TitleM.Sc. Ph.D.
E-mailpzerefos@bioacademy.gr
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue: Urine [eVOC:EV:0000002]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique None
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation Yes
Mass spectrometer
Instrument: Agilent Ion-Trap 6330
Vendor: Agilent
Ionization method ESI
Fragmentation method CID
Mass tolerance used for database searching (MS) 1.5 Da
Mass tolerance used for database searching (MS/MS) 0.7 Da
Database used for searching Swissprot
Search engine used Mascot
Download MS raw dataset  
Download processed files  



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