Experimental Details - HuPA_00696
Experiment type Mass spectrometry
Short description SILAC-based quantitative proteomic analysis of gastric cancer secretome
Experimental description We used a SILAC-based quantitative approach coupled with tandem mass spectrometry to identify differentially expressed proteins Proteins enriched in the cell supernatants were resolved by in-gel and SCX-based fractionation methods. The resulting mass spectral data was searched against the NCBI RefSeq49 human protein database using two different search algorithms Mascot and SEQUEST
Principal Investigator's NameAkhilesh Pandey
AddressInstitute of Bioinformatics
Johns Hopkins University,733 N. Broadway Street, BRB-561
Baltimore, MD 21205 21205
Data submitted byArivusudar Marimuthu
TitleFaculty Scientist
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Cell line: AGS, KatoIII, NCI-N87, SNU-1, SNU-5 , SNU-16 and HFE-145
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique 13C6 R and 13C6 K (13C6 arginine and 13C6 lysine)
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation Reduction of samples was carried out using Dithiothreitol followed by alkylation using iodoacetamide
Mass spectrometer
Instrument: LTQ-Orbitrap Velos
Vendor: Thermo
Ionization method NSI
Fragmentation method HCD
Mass tolerance used for database searching (MS) 20 ppm
Mass tolerance used for database searching (MS/MS) 0.1 Da
Database used for searching Refseq 49
Search engine used Sequest, Mascot
Download MS raw dataset  
Download processed files  

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