HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00697
Experiment type Mass spectrometry
Short description Discovery of potential biomarkers in gastric adenocarcinoma by iTRAQ-based quantitative proteomic approach
Experimental description We have carried out iTRAQ-based proteomic analysis of gastric adenocarcinoma tissue
Principal Investigator's NameAkhilesh Pandey
TitleProfessor
E-mailpandey@jhmi.edu
AddressInstitute of Bioinformatics
733 North Broadway
Baltimore, MD 21205
CountryU.S.A
Data submitted byYashwanth Subbannayya
TitlePh.D. student
E-mailyashwanth@ibioinformatics.org
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue: Stomach [eVOC:EV:0100070]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique None
Protease used Trypsin
Is the sample from in gel No
Reduction and Alkylation Yes
Mass spectrometer
Instrument: Agilent 6520 Accurate-Mass QToF
Vendor: Agilent Technologies
Ionization method Nanospray
Fragmentation method CID
Mass tolerance used for database searching (MS) 100 ppm
Mass tolerance used for database searching (MS/MS) 0.1 Da
Database used for searching Refseq 45
Search engine used Sequest and Mascot
Download MS raw dataset  
Download processed files  



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