HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00698
Experiment type Mass spectrometry
Short description Proteomic analysis of osteoarthritis synovial fluid
Experimental description Synovial fluid from osteoarthritis (OA) patients was pooled and subjected to depletion using multiple affinity removal system to remove the abundant proteins. The depleted protein was then fractionated by SDS-PAGE, SCX and Offgel. In addition, lectin affinity chromatography of OA synovial fluid was carried out to enrich the glycoproteins. Lectin enriched glycoprotein was fractionated by SDS-PAGE and SCX. All the fractions were analyzed on the high resolution Orbitrap Velos mass spectrometer.
Principal Investigator's NameAkhilesh Pandey
TitleProfessor
E-mailpandey@jhmi.edu
AddressInstitute of Bioinformatics
560 Broadway Research Building 733 N. Broadway
Baltimore, MD 21205
CountryUS
Lab URLhttp://www.ibioinformatics.org
Data submitted byLavanya Balakrishnan
TitlePh.D. Student
E-maillavanya@ibioinformatics.org
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue: Synovial fluid [eVOC:EV:0100429]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique None
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation Reduction of samples was carried out using Dithiothreitol followed by alkylation using iodoacetamide
Mass spectrometer
Instrument: LTQ-Orbitrap Velos
Vendor: Thermo Scientific
Ionization method NSI
Fragmentation method HCD
Mass tolerance used for database searching (MS) 20 ppm
Mass tolerance used for database searching (MS/MS) 0.1 Da
Database used for searching Refseq 49
Search engine used Sequest, Mascot
Download MS raw dataset  
Download processed files  



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