Experimental Details - HuPA_00699
Experiment type Mass spectrometry
Short description Quantitative glycoprotein profiling of rheumatoid arthritis and spondyloarthropathy
Experimental description Combination of mulitple lectins with different specifities were used to enrich glycoproteins from synovial fluids of rheumatoid arthritis (RA) and Spondyloarthropathy (SpA). The differentially expressed proteins among the diseases were profiled by iTRAQ labeling (RA with 116 and SpA with 117) followed by analyzing samples on a high resolution LTQ Orbitrap Velos.
Principal Investigator's NameMItali Bhattacharjee
AddressInstitute of Bioinformatics
ITPL Discoverer
Bangalore, Karnataka 560066
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue: Synovial fluid [eVOC:EV:0100429]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique iTRAQ
Protease used Trypsin
Is the sample from in gel No
Reduction and Alkylation Yes
Mass spectrometer
Instrument: LTQ orbitrap Velos
Vendor: Thermo Scientific
Ionization method nano ESI
Fragmentation method HCD
Mass tolerance used for database searching (MS) 20ppm
Mass tolerance used for database searching (MS/MS) 0.1 Da
Database used for searching RefSeq 45
Search engine used Sequest and Mascot
Download MS raw dataset  
Download processed files  

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