HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00700
Experiment type Mass spectrometry
Short description Protein expression profiling of normal human placenta using two-dimensional gel electrophoresis
Experimental description Protein expression profiling of normal human placenta using two-dimensional gel electrophoresis
Principal Investigator's NameRavi Sirdeshmukh
TitleDr.
E-mailravi@ccmb.res.in
AddressCentre for Cellular and Molecular Biology
Centre for Cellular and Molecular Biology
Hyderabad, Andhra Pradesh 500007
CountryIndia
Data submitted byPoonam Gautam
TitleReseach Associate
E-mailpoonam@ccmb.res.in
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue: Placenta [eVOC:EV:0100119]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation Yes, Dithiothritol and Iodoacetamide
Mass spectrometer
Instrument: 4800 MALDI-TOF/TOF Proteomics Analyzer
Vendor: Applied Biosystems, Framingham, MA
Ionization method MALDI
Fragmentation method CID
Mass tolerance used for database searching (MS) 50 ppm
Mass tolerance used for database searching (MS/MS) 0.2 Da
Database used for searching Swissprot
Search engine used Mascot
Download MS raw dataset  
Download processed files  



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