HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00703
Experiment type Mass spectrometry
Short description Proteins with altered levels in serum from acute ischemic stroke patients as revealed by iTRAQ-based differential/quantitative proteomic analysis
Experimental description Proteins with altered levels in serum from acute ischemic stroke patients as revealed by iTRAQ-based differential/quantitative proteomic analysis
Principal Investigator's NameRakesh Sharma
TitleResearch Associate
E-mailsharmabiogeny@gmail.com
AddressNIMHANS
Hosur road
Bangalore, Karnataka 570005
CountryIndia
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue: Serum [eVOC:EV:0100489]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique 4-plex iTRAQ
Protease used Trypsin
Is the sample from in gel No
Reduction and Alkylation Yes
Mass spectrometer
Instrument: LTQ orbitrap Velos
Vendor: Thermo Scientific
Ionization method nano ESI
Fragmentation method HCD
Mass tolerance used for database searching (MS) 20ppm
Mass tolerance used for database searching (MS/MS) 0.1 Da
Database used for searching RefSeq 52
Search engine used Sequest and Mascot
Download MS raw dataset  
Download processed files  



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