HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00709
Experiment type Mass spectrometry
Short description Analysis of urine low MW proteome (peptidome)
Experimental description Analysis of urine low MW proteome (peptidome). Urine samples derived from Bladder Cancer patients and benign controls were analyzed using a comprehensive fractionation strategy, involving immobilized nickel affinity chromatography. IMAC eluates were then analyzed using high resolution LC-MS/MS analysis for their content in naturally occurring urinary peptides.
Principal Investigator's NameAntonia Vlahou
TitleDirector of Proteomics Laboratory
E-mailvlahoua@bioacademy.gr
AddressBiomedical Research Foundation, Academy of Athens
4 Soranou Efesiou
Athens, Athens 11527
CountryGreece
Data submitted byMaria Frantzi
TitlePh.D. student
E-mailmfrantzi@bioacademy.gr
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue: Urine [eVOC:EV:0000002]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique No
Protease used No
Is the sample from in gel No
Reduction and Alkylation No
Mass spectrometer
Instrument: Orbitrap LTQ-Velos FTMS
Vendor: Thermo Fisher, Hemel, UK
Ionization method ESI
Fragmentation method HCD
Mass tolerance used for database searching (MS) 10 ppm
Mass tolerance used for database searching (MS/MS) 0.1 Da
Database used for searching Uniprot: version 01052012 of human taxonomy
Search engine used SEQUEST
Download MS raw dataset  
Download processed files  



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