Experimental Details - HuPA_00851
Experiment type Mass spectrometry
Short description Quantitative proteomic analysis of cryptococcal meningitis
Experimental description Quantitative proteomics of cryptococcal meningitis brain tissues and control brain tissues was carried out to study the host factors that are activated and differentially regulated in response to infection. Proteins isolated from infected brain tissues and control brain tissues were subjected to trypsin digestion and then labeled using 4-plex iTRAQ labels. iTRAQ labeled peptides were subjected to fractionation by SCX. All the fractions were analyzed on the high resolution Orbitrap Velos mass spectrometer.
Principal Investigator's NameDr. Keshava Prasad
TitleFaculty Scientist
AddressInstitute of Bioinformatics
Discoverer Building, 7th Floor
Whitefield, Bangalor, Karnataka 560 066
Data submitted byLakshmi Dhevi N Selvan
TitlePh.D. student
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue: Human brain tissues
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique 4plex iTRAQ
Protease used Trypsin
Is the sample from in gel No
Reduction and Alkylation Yes
Mass spectrometer
Instrument: LTQ-Orbitrap Velos
Vendor: Thermo Scientific
Ionization method Nanospray
Fragmentation method HCD
Mass tolerance used for database searching (MS) 20 ppm
Mass tolerance used for database searching (MS/MS) 0.1 Da
Database used for searching Human RefSeq 52
Search engine used Sequest
Download MS raw dataset  
Download processed files  

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