HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00852
Experiment type Mass spectrometry
Short description Synovial fluid proteome of rheumatoid arthritis
Experimental description A global human synovial proteome was catalogued by MS. Depletion of abundant proteins and multi- lectin affinity- based glycoprotein enrichment were the two modes of protein enrichment implemented. Tryptic peptide fractions were then subjected to 2D nanoLC cloupled to an Orbitrap mass spectrometer (MS)
Principal Investigator's NameMItali Bhattacharjee
TitlePh.D.
E-mailmitalibhattacharjee@gmail.com
AddressInstitute of Bioinformatics
ITPL Discoverer
Bangalore, Karnataka 560066
CountryIndia
Lab URLhttp://www.ibioinformatics.org
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue: Synovial fluid [eVOC:EV:0100429]
Cell line:
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique
Protease used Trypsin
Is the sample from in gel Yes
Reduction and Alkylation Yes
Mass spectrometer
Instrument: orbitrap XL
Vendor: Thermo Scientific
Ionization method nano ESI
Fragmentation method CID
Mass tolerance used for database searching (MS) 20ppm
Mass tolerance used for database searching (MS/MS) 0.8 Da
Database used for searching RefSeq 50
Search engine used Sequest and Mascot
Download MS raw dataset  
Download processed files  



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