HUMAN PROTEINPEDIA 


Experimental Details - HuPA_00803
Experiment type Mass spectrometry
Short description Gallbladder cancer total proteome
Experimental description A mass spectrometry-based quantitative total proteome analysis of gallbladder cancer cell lines was carrired out. The proteins were extracted, reduced, alkylated and digested using trypsin. The tryptic peptides were then labeled using 8-plex iTRAQ reagents and fractionated using strong cation exchange chromatography. The fractions were then subjected to LC-MS/MS analysis.
Principal Investigator's NameAkhilesh Pandey
TitleProfessor
E-mailpandey@jhmi.edu
AddressJohns Hopkins University
733 North Broadway
Baltimore, MD 21205
CountryUSA
Lab URLhttp://pandeylab.igm.jhmi.edu
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Tissue:
Cell line: Gallbladder cancer cell lines
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique 8-plex iTRAQ
Protease used Trypsin
Is the sample from in gel No
Reduction and Alkylation Yes
Mass spectrometer
Instrument: LTQ-Orbitrap Velos
Vendor: Thermo Scientific
Ionization method nano ESI
Fragmentation method HCD
Mass tolerance used for database searching (MS) 20ppm
Mass tolerance used for database searching (MS/MS) 0.05 Da
Database used for searching RefSeq 65
Search engine used Sequest and Mascot
Download MS raw dataset  
Download processed files  



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