Experimental Details - HuPA_00804
Experiment type Mass spectrometry
Short description ITRAQ-based analysis of normal oral keratinocytes and normal oral keratinocytes chronically treated with chewing tobacco
Experimental description LC-MS/MS analysis of normal oral keratinocytes chronically exposed to chewing tobacco and untreated cells
Principal Investigator's NameAkhilesh Pandey
AddressJohns Hopkins University
733 North Broadway
Baltimore, MD 21205
Published/Unpublished Unpublished
Journal name Not applicable
PubMed ID Not applicable
Sample source
Cell line: Human cell line
Source organism Homo sapiens [Taxonomy:9606]
Labeling technique iTRAQ
Protease used Trypsin
Is the sample from in gel No
Reduction and Alkylation Yes
Mass spectrometer
Instrument: LTQ-Orbitrap Velos mass spectrometer
Vendor: Thermo Fischer Scientific
Ionization method nano ESI
Fragmentation method HCD
Mass tolerance used for database searching (MS) 20ppm
Mass tolerance used for database searching (MS/MS) 0.05 Da
Database used for searching RefSeq 65
Search engine used Sequest and Mascot
Download MS raw dataset  
Download processed files  

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